# 0.4 Connecting lines

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## Rationale

OK, so now we have a (relatively) clean image of the cell’s borders. Can we run an edge detector now you ask…? Hold your horses, Champ, we still have a ways to go…

Although it may make sense to run an edge detector at this point, seeing as we’ve got ourselves an image where the cell borders are definitely distinguishable, there is still a problem. Edge detectors look for changes in the gradient. Since our image was acquired utilizing optical fibers, our cell borders are not really solid lines quite yet; they are merely groups of small dots which, together, make up the cell borders. If an edge detector were utilized at this point, it would pick up each fiber optic probe, rather than the cell border we want. There no need to despair; there is a solution for all this!

## Mode filtering

Although it may not be as simple as those connect-the-dots books we’re used to, mode filtering can be an effective method of forming solid cell borders. The basic idea is this: if we used sliding neighborhood operations again, but rather than look for the mean, look for the mode, we may be able to connect all our little dots together! Why is this? Well, the mode filter we implemented works like this:

• Take a neighborhood of size [N M]
• Find the mean of the entries in the matrix.
• Since the image is in black and white, if the mean is greater than .5, then there are more ones, otherwise, there are more zeros. This is basically determining the mode of the neighborhood.
• Thus, if the mode is 1, set the pixel to 1. If the mode is 0, set the pixel to 0.

How does this connect the lines? Think about this: we want to establish solid cell borders by turning ON the black pixels which are part of the cell’s border. Thus, we want to turn ON black pixels which are near groups of white pixels which make up the cell borders and keep black pixels which are not part of the cell borders (in areas with relatively few white pixels) OFF. Taking the mode of each pixel’s neighborhood will accomplish this since black pixels near large groups of white pixels will be turned on and the following image shows what begins to look like solid cell borders… (Figure 1)

can someone help me with some logarithmic and exponential equations.
20/(×-6^2)
Salomon
okay, so you have 6 raised to the power of 2. what is that part of your answer
I don't understand what the A with approx sign and the boxed x mean
it think it's written 20/(X-6)^2 so it's 20 divided by X-6 squared
Salomon
I'm not sure why it wrote it the other way
Salomon
I got X =-6
Salomon
ok. so take the square root of both sides, now you have plus or minus the square root of 20= x-6
oops. ignore that.
so you not have an equal sign anywhere in the original equation?
Commplementary angles
hello
Sherica
im all ears I need to learn
Sherica
right! what he said ⤴⤴⤴
Tamia
what is a good calculator for all algebra; would a Casio fx 260 work with all algebra equations? please name the cheapest, thanks.
a perfect square v²+2v+_
kkk nice
algebra 2 Inequalities:If equation 2 = 0 it is an open set?
or infinite solutions?
Kim
The answer is neither. The function, 2 = 0 cannot exist. Hence, the function is undefined.
Al
y=10×
if |A| not equal to 0 and order of A is n prove that adj (adj A = |A|
rolling four fair dice and getting an even number an all four dice
Kristine 2*2*2=8
Differences Between Laspeyres and Paasche Indices
No. 7x -4y is simplified from 4x + (3y + 3x) -7y
is it 3×y ?
J, combine like terms 7x-4y
im not good at math so would this help me
yes
Asali
I'm not good at math so would you help me
Samantha
what is the problem that i will help you to self with?
Asali
how do you translate this in Algebraic Expressions
Need to simplify the expresin. 3/7 (x+y)-1/7 (x-1)=
. After 3 months on a diet, Lisa had lost 12% of her original weight. She lost 21 pounds. What was Lisa's original weight?
what's the easiest and fastest way to the synthesize AgNP?
China
Cied
types of nano material
I start with an easy one. carbon nanotubes woven into a long filament like a string
Porter
many many of nanotubes
Porter
what is the k.e before it land
Yasmin
what is the function of carbon nanotubes?
Cesar
what is nanomaterials​ and their applications of sensors.
what is nano technology
what is system testing?
preparation of nanomaterial
Yes, Nanotechnology has a very fast field of applications and their is always something new to do with it...
what is system testing
what is the application of nanotechnology?
Stotaw
In this morden time nanotechnology used in many field . 1-Electronics-manufacturad IC ,RAM,MRAM,solar panel etc 2-Helth and Medical-Nanomedicine,Drug Dilivery for cancer treatment etc 3- Atomobile -MEMS, Coating on car etc. and may other field for details you can check at Google
Azam
anybody can imagine what will be happen after 100 years from now in nano tech world
Prasenjit
after 100 year this will be not nanotechnology maybe this technology name will be change . maybe aftet 100 year . we work on electron lable practically about its properties and behaviour by the different instruments
Azam
name doesn't matter , whatever it will be change... I'm taking about effect on circumstances of the microscopic world
Prasenjit
how hard could it be to apply nanotechnology against viral infections such HIV or Ebola?
Damian
silver nanoparticles could handle the job?
Damian
not now but maybe in future only AgNP maybe any other nanomaterials
Azam
can nanotechnology change the direction of the face of the world
At high concentrations (>0.01 M), the relation between absorptivity coefficient and absorbance is no longer linear. This is due to the electrostatic interactions between the quantum dots in close proximity. If the concentration of the solution is high, another effect that is seen is the scattering of light from the large number of quantum dots. This assumption only works at low concentrations of the analyte. Presence of stray light.
the Beer law works very well for dilute solutions but fails for very high concentrations. why?
how did you get the value of 2000N.What calculations are needed to arrive at it
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