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Edge detection

Canny edge detector

We use the Canny Edge Detector to (hopefully) obtain sharp edges for our image now that we have “blurred” together the dots which form the cell edges. The Canny edge detector first takes a gradient of the image. A gradient magnitude, as well as the edge direction (which way the gradient is increasing) can then be found. The Canny edge detector does this by using four different filters which can detect horizontal, vertical and diagonal edges. For each pixel, the filter which outputs the largest response can be found and the direction determined.

Distance matrix

Once we have run our image through the Canny edge detector, a distance filter is used to connect all remaining lines in a cell’s border. The distance function calculates the Euclidean distance between each pixel and the nearest pixel which is on. The output of the distance function is a matrix having the same dimensions as the image matrix. The entries correspond to the distances; for example, if pixel (i,j) was a distance x away from the nearest on pixel, the (i,j) entry in the distance matrix will be x.

After the distance matrix is found, an iterative method is used to go through each entry of the matrix. A threshold distance is determined and every entry of the distance matrix which falls under the threshold is mapped to one, and every entry which falls above is mapped to zero. What exactly is this doing? Well, if a pixel is close enough to an on pixel, then we assume it is part of the cell’s border, and thus must be turned on as well. The distance function and subsequent thresholding is performed multiple times, with the threshold getting small and small each time until the cell borders are completely continuous. The result is shown below.

Questions & Answers

Do somebody tell me a best nano engineering book for beginners?
s. Reply
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Devang Reply
are you nano engineer ?
s.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Harper
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SUYASH Reply
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SUYASH
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s. Reply
of graphene you mean?
Ebrahim
or in general
Ebrahim
in general
s.
Graphene has a hexagonal structure
tahir
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Cied
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Cied
types of nano material
abeetha Reply
I start with an easy one. carbon nanotubes woven into a long filament like a string
Porter
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Porter
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Yasmin
what is the function of carbon nanotubes?
Cesar
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Uday
what is nanomaterials​ and their applications of sensors.
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what is nano technology
Sravani Reply
what is system testing?
AMJAD
preparation of nanomaterial
Victor Reply
Yes, Nanotechnology has a very fast field of applications and their is always something new to do with it...
Himanshu Reply
good afternoon madam
AMJAD
what is system testing
AMJAD
what is the application of nanotechnology?
Stotaw
In this morden time nanotechnology used in many field . 1-Electronics-manufacturad IC ,RAM,MRAM,solar panel etc 2-Helth and Medical-Nanomedicine,Drug Dilivery for cancer treatment etc 3- Atomobile -MEMS, Coating on car etc. and may other field for details you can check at Google
Azam
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Prasenjit
after 100 year this will be not nanotechnology maybe this technology name will be change . maybe aftet 100 year . we work on electron lable practically about its properties and behaviour by the different instruments
Azam
name doesn't matter , whatever it will be change... I'm taking about effect on circumstances of the microscopic world
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silver nanoparticles could handle the job?
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not now but maybe in future only AgNP maybe any other nanomaterials
Azam
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Uday
I'm interested in Nanotube
Uday
this technology will not going on for the long time , so I'm thinking about femtotechnology 10^-15
Prasenjit
can nanotechnology change the direction of the face of the world
Prasenjit Reply
At high concentrations (>0.01 M), the relation between absorptivity coefficient and absorbance is no longer linear. This is due to the electrostatic interactions between the quantum dots in close proximity. If the concentration of the solution is high, another effect that is seen is the scattering of light from the large number of quantum dots. This assumption only works at low concentrations of the analyte. Presence of stray light.
Ali Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
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