<< Chapter < Page Chapter >> Page >

Edge detection

Canny edge detector

We use the Canny Edge Detector to (hopefully) obtain sharp edges for our image now that we have “blurred” together the dots which form the cell edges. The Canny edge detector first takes a gradient of the image. A gradient magnitude, as well as the edge direction (which way the gradient is increasing) can then be found. The Canny edge detector does this by using four different filters which can detect horizontal, vertical and diagonal edges. For each pixel, the filter which outputs the largest response can be found and the direction determined.

Distance matrix

Once we have run our image through the Canny edge detector, a distance filter is used to connect all remaining lines in a cell’s border. The distance function calculates the Euclidean distance between each pixel and the nearest pixel which is on. The output of the distance function is a matrix having the same dimensions as the image matrix. The entries correspond to the distances; for example, if pixel (i,j) was a distance x away from the nearest on pixel, the (i,j) entry in the distance matrix will be x.

After the distance matrix is found, an iterative method is used to go through each entry of the matrix. A threshold distance is determined and every entry of the distance matrix which falls under the threshold is mapped to one, and every entry which falls above is mapped to zero. What exactly is this doing? Well, if a pixel is close enough to an on pixel, then we assume it is part of the cell’s border, and thus must be turned on as well. The distance function and subsequent thresholding is performed multiple times, with the threshold getting small and small each time until the cell borders are completely continuous. The result is shown below.

Questions & Answers

what does nano mean?
Anassong Reply
nano basically means 10^(-9). nanometer is a unit to measure length.
do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
for teaching engĺish at school how nano technology help us
Do somebody tell me a best nano engineering book for beginners?
s. Reply
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
what's the easiest and fastest way to the synthesize AgNP?
Damian Reply
types of nano material
abeetha Reply
I start with an easy one. carbon nanotubes woven into a long filament like a string
many many of nanotubes
what is the k.e before it land
what is the function of carbon nanotubes?
I'm interested in nanotube
what is nanomaterials​ and their applications of sensors.
Ramkumar Reply
what is nano technology
Sravani Reply
what is system testing?
preparation of nanomaterial
Victor Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
Privacy Information Security Software Version 1.1a
Got questions? Join the online conversation and get instant answers!
QuizOver.com Reply

Get the best Algebra and trigonometry course in your pocket!

Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
Google Play and the Google Play logo are trademarks of Google Inc.

Notification Switch

Would you like to follow the 'Detection of cell boundaries in optical fiber probe images' conversation and receive update notifications?