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By the end of this section, you will be able to:
  • Explain how a mass spectrometer works to separate charges
  • Explain how a cyclotron works

Being able to manipulate and sort charged particles allows deeper experimentation to understand what matter is made of. We first look at a mass spectrometer to see how we can separate ions by their charge-to-mass ratio. Then we discuss cyclotrons as a method to accelerate charges to very high energies.

Mass spectrometer

The mass spectrometer    is a device that separates ions according to their charge-to-mass ratios. One particular version, the Bainbridge mass spectrometer, is illustrated in [link] . Ions produced at a source are first sent through a velocity selector, where the magnetic force is equally balanced with the electric force. These ions all emerge with the same speed v = E / B since any ion with a different velocity is deflected preferentially by either the electric or magnetic force, and ultimately blocked from the next stage. They then enter a uniform magnetic field B 0 where they travel in a circular path whose radius R is given by [link] . The radius is measured by a particle detector located as shown in the figure.

A schematic of the Bainbridge mass spectrometer. Charged particles moving down enter a region with electric field E pointing to the left and magnetic field B pointing into the page. The particle path continues in a straight line until it enters a region with no electric field. The magnetic field here is uniform, into the page, and magnitude B naught. The particle path in this region curves in a counterclockwise circle of radius R until it hits a particle detector.
A schematic of the Bainbridge mass spectrometer, showing charged particles leaving a source, followed by a velocity selector where the electric and magnetic forces are balanced, followed by a region of uniform magnetic field where the particle is ultimately detected.

The relationship between the charge-to-mass ratio q / m and the radius R is determined by combining [link] and [link] :

q m = E B B 0 R .

Since most ions are singly charged ( q = 1.6 × 10 −19 C ) , measured values of R can be used with this equation to determine the mass of ions. With modern instruments, masses can be determined to one part in 10 8 .

An interesting use of a spectrometer is as part of a system for detecting very small leaks in a research apparatus. In low-temperature physics laboratories, a device known as a dilution refrigerator uses a mixture of He-3, He-4, and other cryogens to reach temperatures well below 1 K. The performance of the refrigerator is severely hampered if even a minute leak between its various components occurs. Consequently, before it is cooled down to the desired temperature, the refrigerator is subjected to a leak test. A small quantity of gaseous helium is injected into one of its compartments, while an adjacent, but supposedly isolated, compartment is connected to a high-vacuum pump to which a mass spectrometer is attached. A heated filament ionizes any helium atoms evacuated by the pump. The detection of these ions by the spectrometer then indicates a leak between the two compartments of the dilution refrigerator.

In conjunction with gas chromatography, mass spectrometers are used widely to identify unknown substances. While the gas chromatography portion breaks down the substance, the mass spectrometer separates the resulting ionized molecules. This technique is used with fire debris to ascertain the cause, in law enforcement to identify illegal drugs, in security to identify explosives, and in many medicinal applications.

Questions & Answers

how does Neisseria cause meningitis
Nyibol Reply
what is microbiologist
Muhammad Reply
what is errata
Muhammad
is the branch of biology that deals with the study of microorganisms.
Ntefuni Reply
What is microbiology
Mercy Reply
studies of microbes
Louisiaste
when we takee the specimen which lumbar,spin,
Ziyad Reply
How bacteria create energy to survive?
Muhamad Reply
Bacteria doesn't produce energy they are dependent upon their substrate in case of lack of nutrients they are able to make spores which helps them to sustain in harsh environments
_Adnan
But not all bacteria make spores, l mean Eukaryotic cells have Mitochondria which acts as powerhouse for them, since bacteria don't have it, what is the substitution for it?
Muhamad
they make spores
Louisiaste
what is sporadic nd endemic, epidemic
Aminu Reply
the significance of food webs for disease transmission
Abreham
food webs brings about an infection as an individual depends on number of diseased foods or carriers dully.
Mark
explain assimilatory nitrate reduction
Esinniobiwa Reply
Assimilatory nitrate reduction is a process that occurs in some microorganisms, such as bacteria and archaea, in which nitrate (NO3-) is reduced to nitrite (NO2-), and then further reduced to ammonia (NH3).
Elkana
This process is called assimilatory nitrate reduction because the nitrogen that is produced is incorporated in the cells of microorganisms where it can be used in the synthesis of amino acids and other nitrogen products
Elkana
Examples of thermophilic organisms
Shu Reply
Give Examples of thermophilic organisms
Shu
advantages of normal Flora to the host
Micheal Reply
Prevent foreign microbes to the host
Abubakar
they provide healthier benefits to their hosts
ayesha
They are friends to host only when Host immune system is strong and become enemies when the host immune system is weakened . very bad relationship!
Mark
what is cell
faisal Reply
cell is the smallest unit of life
Fauziya
cell is the smallest unit of life
Akanni
ok
Innocent
cell is the structural and functional unit of life
Hasan
is the fundamental units of Life
Musa
what are emergency diseases
Micheal Reply
There are nothing like emergency disease but there are some common medical emergency which can occur simultaneously like Bleeding,heart attack,Breathing difficulties,severe pain heart stock.Hope you will get my point .Have a nice day ❣️
_Adnan
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Innocent
I think infection prevention and control is the avoidance of all things we do that gives out break of infections and promotion of health practices that promote life
Lubega
Heyy Lubega hussein where are u from?
_Adnan
en français
Adama
which site have a normal flora
ESTHER Reply
Many sites of the body have it Skin Nasal cavity Oral cavity Gastro intestinal tract
Safaa
skin
Asiina
skin,Oral,Nasal,GIt
Sadik
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all
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by fussion
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what are the advantages of normal Flora to the host
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Shelly Reply
part of a tissue or an organ being wounded or bruised.
Wilfred
what term is used to name and classify microorganisms?
Micheal Reply
Binomial nomenclature
adeolu
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Source:  OpenStax, University physics volume 2. OpenStax CNX. Oct 06, 2016 Download for free at http://cnx.org/content/col12074/1.3
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