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Image depicts a man using scanning electron microscope.
An electron microscope has the capability to image individual atoms on a material. The microscope uses vacuum technology, sophisticated detectors and state of the art image processing software. (credit: Dave Pape)
The figure gives an artist's view of different stages of meiosis.
The image shows a sequence of events that takes place during meiosis. (credit: PatríciaR, Wikimedia Commons; National Center for Biotechnology Information)

Take-home experiment: make a lens

Look through a clear glass or plastic bottle and describe what you see. Now fill the bottle with water and describe what you see. Use the water bottle as a lens to produce the image of a bright object and estimate the focal length of the water bottle lens. How is the focal length a function of the depth of water in the bottle?

Section summary

  • The microscope is a multiple-element system having more than a single lens or mirror.
  • Many optical devices contain more than a single lens or mirror. These are analysed by considering each element sequentially. The image formed by the first is the object for the second, and so on. The same ray tracing and thin lens techniques apply to each lens element.
  • The overall magnification of a multiple-element system is the product of the magnifications of its individual elements. For a two-element system with an objective and an eyepiece, this is
    m = m o m e ,
    where m o size 12{m rSub { size 8{o} } } {} is the magnification of the objective and m e size 12{m rSub { size 8{e} } } {} is the magnification of the eyepiece, such as for a microscope.
  • Microscopes are instruments for allowing us to see detail we would not be able to see with the unaided eye and consist of a range of components.
  • The eyepiece and objective contribute to the magnification. The numerical aperture ( NA ) size 12{ \( ital "NA" \) } {} of an objective is given by
    NA = n sin α size 12{ ital "NA"=n"sin"α} {}
    where n size 12{n} {} is the refractive index and α size 12{α} {} the angle of acceptance.
  • Immersion techniques are often used to improve the light gathering ability of microscopes. The specimen is illuminated by transmitted, scattered or reflected light though a condenser.
  • The f /# size 12{ {f} slash {#} } {} describes the light gathering ability of a lens. It is given by
    f /# = f D 1 2 NA .

Conceptual questions

Geometric optics describes the interaction of light with macroscopic objects. Why, then, is it correct to use geometric optics to analyse a microscope’s image?

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The image produced by the microscope in [link] cannot be projected. Could extra lenses or mirrors project it? Explain.

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Why not have the objective of a microscope form a case 2 image with a large magnification? (Hint: Consider the location of that image and the difficulty that would pose for using the eyepiece as a magnifier.)

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What advantages do oil immersion objectives offer?

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How does the NA size 12{ ital "NA"} {} of a microscope compare with the NA size 12{ ital "NA"} {} of an optical fiber?

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Problem exercises

A microscope with an overall magnification of 800 has an objective that magnifies by 200. (a) What is the magnification of the eyepiece? (b) If there are two other objectives that can be used, having magnifications of 100 and 400, what other total magnifications are possible?

(a) 4.00

(b) 1600

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(a) What magnification is produced by a 0.150 cm focal length microscope objective that is 0.155 cm from the object being viewed? (b) What is the overall magnification if an eyepiece (one that produces a magnification of 8.00) is used?

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(a) Where does an object need to be placed relative to a microscope for its 0.500 cm focal length objective to produce a magnification of –400 ? (b) Where should the 5.00 cm focal length eyepiece be placed to produce a further fourfold (4.00) magnification?

(a) 0.501 cm

(b) Eyepiece should be 204 cm behind the objective lens.

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You switch from a 1.40 NA 60 × oil immersion objective to a 1.40 NA 60 × oil immersion objective. What are the acceptance angles for each? Compare and comment on the values. Which would you use first to locate the target area on your specimen?

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An amoeba is 0.305 cm away from the 0.300 cm focal length objective lens of a microscope. (a) Where is the image formed by the objective lens? (b) What is this image’s magnification? (c) An eyepiece with a 2.00 cm focal length is placed 20.0 cm from the objective. Where is the final image? (d) What magnification is produced by the eyepiece? (e) What is the overall magnification? (See [link] .)

(a) +18.3 cm (on the eyepiece side of the objective lens)

(b) -60.0

(c) -11.3 cm (on the objective side of the eyepiece)

(d) +6.67

(e) -400

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You are using a standard microscope with a 0.10 NA 4 × objective and switch to a 0.65 NA 40 × objective. What are the acceptance angles for each? Compare and comment on the values. Which would you use first to locate the target area on of your specimen? (See [link] .)

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Unreasonable Results

Your friends show you an image through a microscope. They tell you that the microscope has an objective with a 0.500 cm focal length and an eyepiece with a 5.00 cm focal length. The resulting overall magnification is 250,000. Are these viable values for a microscope?

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Questions & Answers

Electric current is the flow of electrons
Kelly Reply
is there really flow of electrons exist?
babar
Yes It exists
Cffrrcvccgg
explain plz how electrons flow
babar
if electron flows from where first come and end the first one
babar
an electron will flow accross a conductor because or when it posseses kinectic energy
Cffrrcvccgg
electric means the flow heat current.
Serah Reply
electric means the flow of heat current in a circuit.
Serah
What is electric
Manasseh Reply
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ghulam
electric means the flow of heat current in a circuit.
Serah
a boy cycles continuously through a distance of 1.0km in 5minutes. calculate his average speed in ms-1(meter per second). how do I solve this
Jenny Reply
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PhysicswithMrV
why we cannot use DC instead of AC in a transformer
kusshaf Reply
becuse the d .c cannot travel for long distance trnsmission
ghulam
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Chiwetalu Reply
branch of science which deals with matter energy and their relationship between them
ghulam
Life science
the
what is heat and temperature
Kazeem Reply
how does sound affect temperature
Clement Reply
sound is directly proportional to the temperature.
juny
how to solve wave question
Wisdom Reply
I would like to know how I am not at all smart when it comes to math. please explain so I can understand. sincerly
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Just know d relationship btw 1)wave length 2)frequency and velocity
Talhatu
First of all, you are smart and you will get it👍🏽... v = f × wavelength see my youtube channel: "mathwithmrv" if you want to know how to rearrange equations using the balance method
PhysicswithMrV
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Beatrax
thanks dear
Chuks
hi pls help me with this question A ball is projected vertically upwards from the top of a tower 60m high with a velocity of 30ms1.what is the maximum height above the ground level?how long does it take to reach the ground level?
mahmoud
please guys help, what is the difference between concave lens and convex lens
Vincent Reply
convex lens brings rays of light to a focus while concave diverges rays of light
Christian
for mmHg to kPa yes
Matthew
it depends on the size
Matthew Reply
please what is concave lens
Vincent
a lens which diverge the ray of light
rinzuala
concave diverges light
Matthew
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Vincent
A diverging lens
Yusuf
What is isotope
Yusuf
each of two or more forms of the same element that contain equal numbers of protons but different numbers of neutrons in their nuclei, and hence differ in relative atomic mass but not in chemical properties; in particular, a radioactive form of an element. "some elements have only one stable isotope
Karthi
what is wire wound resistors?
Naveedkhan Reply
What are the best colleges to go to for physics
Matthew Reply
I would like to know this too
Trevor
How do I calculate uncertainty in a frequency?
Rebecca Reply
Calculate . ..
Olufunsho
What is light wave
Sakeenah Reply
What is wave
Sakeenah
What is light
Sakeenah
okay
True
explain how neurons communicate feed and stimulate
Jeff
Great science students
Omo
A wave is a disturbance which travels through the medium transferring energy from one form to another without causing any permanent displacement of d medium itself
OGOR
Light is a form o wave
OGOR
Neurons communicate by sending message through nerves in coordination
OGOR
What are petrochemicals, give two examples
OGOR
light has dual nature, particle as well as wave. when we want to explain phenomena like Interference of light, then we consider light as wave.
Lalita
what is it as in the form of it or how to visualize it or what it contains
Matthew
particles of light are like small packets of energy called photons, and flow or motion of photons is wave like
Lalita
light is just the energy of which photons emit
Matthew
the wave is how they travel
Matthew
photons do not emitt energy, they are energy. They are massless particles.
Lalita
a wave is a disturbance through the medium. Have you ever thrown a stone in still water? the disturbance produced travels in form of wave, the wave produced by throwing stone in still water are circular in nature.
Lalita
a photon does contain mass when in motion. it doesnt contain mass when at rest
Matthew
when would it ever be at rest
Bob
a wave is a disturbance of which energy travels
Matthew
that's darkness. darkness has no mass because the photons within in aren't moving or producing energy
Matthew
Hi guys. Please I've been trying to understand the concept of SHM, but it's not been really easy, could someone please explain it to me or suggest a site I could visit? Thank you.
Odo
***google.com/url?sa=t&source=web&rct=j&url=***hyperphysics.phy-astr.gsu.edu/hbase/shm.html&ved=2ahUKEwiwu_PYqfzdAhXOmVkKHXi2CvkQFjAPegQIARAB&usg=AOvVaw0h5IZmrkQK4KtEMygT4ZGK
Matthew
effective mass of photons only comes into picture when we consider it accelerating in gravitational field, mass of photon has no meaning as it is always travelling with speed of light and is never at rest. with that high speed, Energy and momentum are equivalent. and darkness is absense of photons.
Lalita
darkness is absense of light. not the presence of 'resting photons'. photons are never at rest.
Lalita
photons are present in darkness but don't give off any light because they are stationary with no mass or energy. once a force makes them move again they will gain mass and give off light
Matthew
this theory is presented in Einsteins theory of special relativity
Matthew
A.The velocity Vo for the streamline flow of liquid in a small tube depends on the radius r of the tube,the density and the viscosity iter of the liquid .use the dimensional analysis to obtain an expression for the velocity . B.Given that Vo =r square ×p all over 4×iter ×l
True
A.The velocity Vo for the streamline flow of liquid in a small tube depends on the radius r of the tube,the density (rho)and the viscosity (iter)of the liquid. Use the method of dimensional analysis to obtain an expression for the velocity . B.Given that Vo =r square x p all over 4 x iter x l
True
Matthew, photons ARE light. there is no such thing as a photon that isn't moving. in fact the speed they move at is called C (for constant) in physics. through a vacuum they always travel at this speed no matter what. they can not slow down; except in another medium.
Brad
The reason why a photon can go at this speed is BECAUSE it had no mass. nothing can go this speed or faster because it needs to have no mass or negative mass. that's why it's called the constant.
Brad
when a photon hits something that is opaque, this is the only way to "stop"it. it isn't merely stopped but absorbed and turned into heat energy, then the remaining energy is reflected in different wavelengths. that reflection is what we call color. the darker something is, the less photons are ther
Brad
e. complete blackness is the absolute absence of photons altogether. I believe what you're referring to is not speed, but wavelength, which is indirectly proportional to the amount of energy a particular photon is made up of.
Brad
in order for a photon to have zero wavelength, it must (at least theoretically) have infinite energy.
Brad
about mass: you may have photons confused with electrons. elections have a mass so small that people say they are without mass, but they do. it is called electron mass or Me-.
Brad
you may also be getting electrons and photons confused because of the cherenkov effect. that is what happens when a particle travels faster than light IN THAT PARTICULAR MEDIUM. I emphasize that because no other particle besides photons can go the speed of c.
Brad
when a particle goes faster than light in a particular medium, a blue light is emitted, called cherenkov radiation. this is why nuclear reactors glow blue.
Brad
nuclear reactors release so much energy that when they emit electrons, those electrons are given enough energy to go faster than light in that medium (in this case water), releasing blue light. if you put the reactor in air or a vacuum, this effect wouldn't happen because the speed of light in air
Brad
is very close to c, which is the universal speed limit. I'd you did go faster than c, time would go backwards and you would have infinite theoretical mass and probably spagghettify, like with a black hole.
Brad
*if
Brad
*electrons
Brad
light waves can travel through a vacuum, and do not require a medium. In empty space, the wave does not dissipate (grow smaller) no matter how far it travels, because the wave is not interacting with anything else.
Salim
Please is there any instructional material for sounds Waves, Echo, light waves
Salami
how far there is hot topic that is boarding me now
Abraham
linear motion
Ahmed
kinematic
Abraham
tell us about it
Akinsanya
kinematic
Emma
kinematics disscuss the motion without cuases ...
ghulam
wow I like what am seeing here I need someone to brush me up in physics in fact I'll say I know nothing
Godslight
Practice Key Terms 4

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Source:  OpenStax, College physics. OpenStax CNX. Jul 27, 2015 Download for free at http://legacy.cnx.org/content/col11406/1.9
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