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Fluorescence can be induced by many types of energy input. Fluorescent paint, dyes, and even soap residues in clothes make colors seem brighter in sunlight by converting some UV into visible light. X rays can induce fluorescence, as is done in x-ray fluoroscopy to make brighter visible images. Electric discharges can induce fluorescence, as in so-called neon lights and in gas-discharge tubes that produce atomic and molecular spectra. Common fluorescent lights use an electric discharge in mercury vapor to cause atomic emissions from mercury atoms. The inside of a fluorescent light is coated with a fluorescent material that emits visible light over a broad spectrum of wavelengths. By choosing an appropriate coating, fluorescent lights can be made more like sunlight or like the reddish glow of candlelight, depending on needs. Fluorescent lights are more efficient in converting electrical energy into visible light than incandescent filaments (about four times as efficient), the blackbody radiation of which is primarily in the infrared due to temperature limitations.

This atom is excited to one of its higher levels by absorbing a UV photon. It can de-excite in a single step, re-emitting a photon of the same energy, or in several steps. The process is called fluorescence if the atom de-excites in smaller steps, emitting energy different from that which excited it. Fluorescence can be induced by a variety of energy inputs, such as UV, x-rays, and electrical discharge.

The spectacular Waitomo caves on North Island in New Zealand provide a natural habitat for glow-worms. The glow-worms hang up to 70 silk threads of about 30 or 40 cm each to trap prey that fly towards them in the dark. The fluorescence process is very efficient, with nearly 100% of the energy input turning into light. (In comparison, fluorescent lights are about 20% efficient.)

Fluorescence has many uses in biology and medicine. It is commonly used to label and follow a molecule within a cell. Such tagging allows one to study the structure of DNA and proteins. Fluorescent dyes and antibodies are usually used to tag the molecules, which are then illuminated with UV light and their emission of visible light is observed. Since the fluorescence of each element is characteristic, identification of elements within a sample can be done this way.

[link] shows a commonly used fluorescent dye called fluorescein. Below that, [link] reveals the diffusion of a fluorescent dye in water by observing it under UV light.

The image shows fluorescent dye sample in red powder form.
Fluorescein, shown here in powder form, is used to dye laboratory samples. (credit: Benjah-bmm27, Wikimedia Commons)
A beaker of water in which fluorescent powder is added. Near the beaker an ultraviolet light source is placed. In this ultraviolet light, the fluorescent powder in the water glows like green smoke.
Here, fluorescent powder is added to a beaker of water. The mixture gives off a bright glow under ultraviolet light. (credit: Bricksnite, Wikimedia Commons)

Nano-crystals

Recently, a new class of fluorescent materials has appeared—“nano-crystals.” These are single-crystal molecules less than 100 nm in size. The smallest of these are called “quantum dots.” These semiconductor indicators are very small (2–6 nm) and provide improved brightness. They also have the advantage that all colors can be excited with the same incident wavelength. They are brighter and more stable than organic dyes and have a longer lifetime than conventional phosphors. They have become an excellent tool for long-term studies of cells, including migration and morphology. ( [link] .)

Questions & Answers

can some one tell me how v=RW is dimensionally correct?
YAGNAK Reply
ms-1 = m X Hz
babar
What is displacement
Megha Reply
shortest distance b/w two points
bilal
distance+direction
A.d
explain distanace+direction
bilal
the change of postion from one point to another with direction
A.d
if we change thrle direction then displacement is destroy?
bilal
change the direction then?
bilal
what do u mean by i didnt understand bro
A.d
displacement is one dimension...?
bilal
displacement is the total length an object cover from initial to the final with respect to direction as Well as time.
mohammed
thanks
bilal
what are the differences between vector and scalar quantity
Kabba Reply
vector is assigned to those physical quantity that has both direction and magnitude! example velocity ,scalar just has magnitude example Mass of an object. hope it helps
Mudang
velocity is produce in fan...?
bilal Reply
how many electrons are there in 5 microcouloumb
Obed Reply
can a given total amount of mechanical energy be totally converted into heat energy..if so give example
Muhammad Reply
human running
Emmanuel
what is the fumula for calculating specific heat capacity, fusion,fission and vaporization?
Dohn Reply
Q=cm(∆t)
Emmanuel
Q=cm∆T
Muhammad
what is difference b/w vaporization and evaporation
Muhammad
evaporation is the process of extracting moisture while vaporization is process of becoming a vapor or gas
Emmanuel
From a molecular standpoint they are both cooling processes. Also, you may want to explore states of matter😊 #myTwoCents ~Shi~
Shii
cooling is a similarlity in both process I am confused in difference
Muhammad
1- Evaporation is a process where a liquid change to gas without reaching its boiling point. 2- Vaporization is a process where a liquid change to gas after reaching its boiling point. 3- Sublimation is a process where a solid changes into vapour without passing through a liquid state
Victor
I see. Evaporation is a type of vaporization, that occurs on the surface of a liquid as it changes into the gaseous phase before reaching its boiling point. hope that aids
Shii
vaporisation is cooling process while vaporization is heating process
Emmanuel
I mean to write evaporation is an heating process while vaporization is cooling process
Emmanuel
Yea here are two applications. 1- your wet washed clothes dry under the sun, the water EVAPORATES 2- when u are cooking, it reaches a point where u need to add more water because the water you added previously is getting dried. this is VAPORIZATION. Am not sure which is a cooling or heating process
Victor
vaporization occur only when the evaporation get to level where the above cloud is been (saturated) so cooling take place and started to change to liquid (eg rain fall)
Emmanuel
They are both properties of the same process so they're both cooling
Shii
what about sublimation? cooling or heating process?
Victor
exact
Muhammad
evaporation is the increase in kinetic energy of the liquid which can be gone by adding heat
Emmanuel
so its an heating process
Emmanuel
sublimation is when a solid change to gas
Emmanuel
evaporation is very definitely a cooling process. respectfully@Emmanuel when liquid turns to gas it requires more energy from its surroundings, this energy is in the form of heat, and when heat energy leaves the evaporating liquid it leaves it cooler. Thus, cooling process.
Shii
.
Shii
evaporation is very definitely a cooling process. respectfully@Emmanuel
Shii
kk
Emmanuel
You're right @Shi. I get your point
Victor
eascape velocity on the surface of Earth is 11.2 kms-1 the escape velocity on the surface of another planet of same mass as that of Earth but of 1/4 times of radius of Earth is a5.6kms-1 b11.2 kms-1 c22.4kms-1 d5.6ms-1
Muhammad
Emm.. is that a question? or..
Victor
it is McQ
Muhammad
a)5.6km/s
Alvis
c= Q/cm◇T
A.d
it's answer is 22.4
Muhammad
units...
Shii
vital
Shii
the time period of the artificial satellite is given by ?
raza
Why is there no 2nd harmonic in the classical electron orbit?
Shree Reply
how to reform magnet after been demagneted
Inuwa Reply
A petrol engine has a output of 20 kilowatts and uses 4.5 kg of fuel for each hour of running. The energy given out when 1 kg of petrol is burnt is 4.8 × 10 to the power of 7 Joules. a) What is the energy output of the engine every hour? b) What is the energy input of the engine every hour?
Morris Reply
Issac Newton devised a genius way to calculate changing quantities...
Shii
what is the error during taking work done of a body..
Aliyu Reply
what kind of error do you think? and work is held by which force?
Daniela
I am now in this group
smart
theory,laws,principles and what-a-view are not defined. why? you
Douglas Reply
A simple pendulum is used in a physics laboratory experiment to obtain an experimental value for the gravitational acceleration, g . A student measures the length of the pendulum to be 0.510 meters, displaces it 10 o from the equilibrium position, and releases it. Using a s
Emmanuel Reply
so what question are you passing across... sir?
Olalekan
Two masses of 2 kg and 4 kg are held with a compressed spring between them. If the masses are released, the spring will push them away from each other. If the smaller mass moves off with a velocity of 6m/s, what is the stored energy in the spring when it is compressed?
Emmanuel Reply
54 joule
babar
how?
rakesh
Reduce that two body problem into one body problem. Apply potential and k. E formula to get total energy of the system
rakesh
i dont think dere is any potential energy... by d virtue of no height present
Olalekan
there is compressed energy,dats only potential energy na?
rakesh
yes.. but... how will u approach that question without The Height in the question?
Olalekan
Can you explain how you get 54J?
Emmanuel
Because mine is 36J
Emmanuel
got 36J too
Douglas
OK the answer is 54J Babar is correct
Emmanuel
Conservation of Momentum
Emmanuel
woow i see.. can you give the formula for this
joshua
Two masses of 2 kg and 4 kg are held with a compressed spring between them. If the masses are released, the spring will push them away from each other. If the smaller mass moves off with a velocity of 6m/s, what is the stored energy in the spring when it is compressed? Asume there is no external force.
Emmanuel Reply
Please help!
Emmanuel
please help find dy/dx 2x-y/x+y
Inuwa
By using the Quotient Rule dy/dx = 3y/(x +y)²
Emmanuel
3y/(x+y)²
Emmanuel
may be by using MC^2=MC^2 and Total energy=kinetic energy +potential energy so 1st find kinetic energy and den find potential energy which is stored energy
rakesh
i think i m correct
rakesh
But how?
Emmanuel
3y/(x+y)²
Douglas

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Source:  OpenStax, College physics. OpenStax CNX. Jul 27, 2015 Download for free at http://legacy.cnx.org/content/col11406/1.9
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