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What does the amino acid composition analysis show as the most common amino acid in this protein? (Is that unusual?)

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What is the chemical formula for the query protein?

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What is the predicted extinction coefficient at 280 nm, in 6M guanidium HCl, 0.02M phosphate, pH6.5 buffer, assuming all cysteines appear as half cysteines?

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In what way could it be helpful to know the extinction coefficient?

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According to the instability index, is this protein classified as stable or unstable?

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Return again to the ExPASy tools . Notice there are two sections dealing with structure prediction, secondary structure prediction tools and tertiary structureprediction and visualization tools. The secondary structure prediction tools are designed to predict features such as the helical content, the betasheet formations, and the turns, loops, and coil regions within a protein, given the sequence.

Explore the secondary structure tools independently, and submit the diacylglycerol kinase sequenceabove to any of the available secondary structure prediction tools. Most of these tools will email the results, with at leasta 20 minute delay between submission and receipt of results. Forward a results summary to the instructor, outlining the predictions created bythe program of choice.

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Tertiary structure prediction tools match the query sequence with sequences, or partial sequences, of proteins where the 3-Dstructure has been published in the Protein Data Bank (PDB). These tools will produce a model of the query protein by piecing together the structuralregions from the best matches in the PDB, and threading the query sequence through the predicted structure. For more detailed explanations of available3-D structure prediction software, view the Swiss-Model demo page and the Geno3D reference page . Although both of these tools are searching for templates from existing PDB entries, they are doing this in different ways.

What program does Swiss-Model use to match the query sequence with sequences of known structures?

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What program does Geno3D use to match the query sequence with sequences of known structures?

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Notice that the template selection process and the model structure refinement processes are different between these two programs as well.

Finally, in the tertiary structure section of the ExPASy tools page, Swiss PDB Viewer is a graphical tool for the visualization, comparison and analysis of3-D coordinate files. Swiss PDB Viewer can superimpose 3-D structures by finding the rotation and translation that most closely aligns the two proteinstructures. Additionally, the Swiss PDB Viewer will perform amino acid mutations, prediction of hydrogen bonds, and calculation of angles anddistances between atoms. Best of all, Swiss PDB Viewer is freeware and available for many different platforms, including Macintosh, PC, SGI IRIX,and Linux.

View this supplemental SPDBV web page . What other function does Swiss PDB Viewer have, when used in conjunction withother applications such as OpenGL or POV-Ray?

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ExPASy provides a very large library of tools, for proteomics as well as other bioinformatics applications.For those students interested in future research in the field of proteomics,this web server will be an important resource.

Questions & Answers

Do somebody tell me a best nano engineering book for beginners?
s. Reply
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
s.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Harper
how to fabricate graphene ink ?
SUYASH Reply
for screen printed electrodes ?
SUYASH
What is lattice structure?
s. Reply
of graphene you mean?
Ebrahim
or in general
Ebrahim
in general
s.
Graphene has a hexagonal structure
tahir
On having this app for quite a bit time, Haven't realised there's a chat room in it.
Cied
what is biological synthesis of nanoparticles
Sanket Reply
what's the easiest and fastest way to the synthesize AgNP?
Damian Reply
China
Cied
types of nano material
abeetha Reply
I start with an easy one. carbon nanotubes woven into a long filament like a string
Porter
many many of nanotubes
Porter
what is the k.e before it land
Yasmin
what is the function of carbon nanotubes?
Cesar
I'm interested in nanotube
Uday
what is nanomaterials​ and their applications of sensors.
Ramkumar Reply
what is nano technology
Sravani Reply
what is system testing?
AMJAD
preparation of nanomaterial
Victor Reply
Yes, Nanotechnology has a very fast field of applications and their is always something new to do with it...
Himanshu Reply
good afternoon madam
AMJAD
what is system testing
AMJAD
what is the application of nanotechnology?
Stotaw
In this morden time nanotechnology used in many field . 1-Electronics-manufacturad IC ,RAM,MRAM,solar panel etc 2-Helth and Medical-Nanomedicine,Drug Dilivery for cancer treatment etc 3- Atomobile -MEMS, Coating on car etc. and may other field for details you can check at Google
Azam
anybody can imagine what will be happen after 100 years from now in nano tech world
Prasenjit
after 100 year this will be not nanotechnology maybe this technology name will be change . maybe aftet 100 year . we work on electron lable practically about its properties and behaviour by the different instruments
Azam
name doesn't matter , whatever it will be change... I'm taking about effect on circumstances of the microscopic world
Prasenjit
how hard could it be to apply nanotechnology against viral infections such HIV or Ebola?
Damian
silver nanoparticles could handle the job?
Damian
not now but maybe in future only AgNP maybe any other nanomaterials
Azam
Hello
Uday
I'm interested in Nanotube
Uday
this technology will not going on for the long time , so I'm thinking about femtotechnology 10^-15
Prasenjit
can nanotechnology change the direction of the face of the world
Prasenjit Reply
At high concentrations (>0.01 M), the relation between absorptivity coefficient and absorbance is no longer linear. This is due to the electrostatic interactions between the quantum dots in close proximity. If the concentration of the solution is high, another effect that is seen is the scattering of light from the large number of quantum dots. This assumption only works at low concentrations of the analyte. Presence of stray light.
Ali Reply
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Bios 533 bioinformatics. OpenStax CNX. Sep 24, 2008 Download for free at http://cnx.org/content/col10152/1.16
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