<< Chapter < Page Chapter >> Page >

Primary structure

The unique sequence of amino acids in a polypeptide chain is its primary structure    . For example, the pancreatic hormone insulin has two polypeptide chains, A and B, and they are linked together by disulfide bonds. The N terminal amino acid of the A chain is glycine, whereas the C terminal amino acid is asparagine ( [link] ). The sequences of amino acids in the A and B chains are unique to insulin.

The amino acid sequences for the A chain and B chain of bovine insulin are shown. The A chain is 21 amino acids in length, and the B chain is 30 amino acids in length. One disulfide, or SS bond, connects two cysteine residues in the A chain. Two other disulfide linkages connect the A chain to the B chain.
Bovine serum insulin is a protein hormone made of two peptide chains, A (21 amino acids long) and B (30 amino acids long). In each chain, primary structure is indicated by three-letter abbreviations that represent the names of the amino acids in the order they are present. The amino acid cysteine (cys) has a sulfhydryl (SH) group as a side chain. Two sulfhydryl groups can react in the presence of oxygen to form a disulfide (S-S) bond. Two disulfide bonds connect the A and B chains together, and a third helps the A chain fold into the correct shape. Note that all disulfide bonds are the same length, but are drawn different sizes for clarity.

The unique sequence for every protein is ultimately determined by the gene encoding the protein. A change in nucleotide sequence of the gene’s coding region may lead to a different amino acid being added to the growing polypeptide chain, causing a change in protein structure and function. In sickle cell anemia, the hemoglobin β chain (a small portion of which is shown in [link] ) has a single amino acid substitution, causing a change in protein structure and function. Specifically, the amino acid glutamic acid is substituted by valine in the β chain. What is most remarkable to consider is that a hemoglobin molecule is made up of two alpha chains and two beta chains that each consist of about 150 amino acids. The molecule, therefore, has about 600 amino acids. The structural difference between a normal hemoglobin molecule and a sickle cell molecule—which dramatically decreases life expectancy—is a single amino acid of the 600. What is even more remarkable is that those 600 amino acids are encoded by three nucleotides each, and the mutation is caused by a single base change (point mutation), 1 in 1800 bases.

A portion of the hemoglobin amino acid sequence is shown. The normal hemoglobin beta chain has a glutamate at position six. The sickle cell beta chain has a valine at this position.
The beta chain of hemoglobin is 147 residues in length, yet a single amino acid substitution leads to sickle cell anemia. In normal hemoglobin, the amino acid at position seven is glutamate. In sickle cell hemoglobin, this glutamate is replaced by a valine.

Because of this change of one amino acid in the chain, hemoglobin molecules form long fibers that distort the biconcave, or disc-shaped, red blood cells and assume a crescent or “sickle” shape, which clogs arteries ( [link] ). This can lead to myriad serious health problems such as breathlessness, dizziness, headaches, and abdominal pain for those affected by this disease.

This electron micrograph shows red blood cells from a patient with sickle cell anemia. Most of the cells have a normal, disk shape, but about one in five has a sickle shape. A normal blood cell is eight microns across.
In this blood smear, visualized at 535x magnification using bright field microscopy, sickle cells are crescent shaped, while normal cells are disc-shaped. (credit: modification of work by Ed Uthman; scale-bar data from Matt Russell)

Questions & Answers

what is abiotic and biotic factors?
Hira Reply
which of the following shows the correct sequence of the cell cycle
Kameishia Reply
who is name virus
Shivam Reply
centromere consist of
meeting point of two chromatids
Explain the function of nematocysts in cnidarians?
Israel Reply
The nemotocyst is used by Cnidarians (hydra, jellyfish, sea anemones) to sting their prey and any threatening enemy.
photosynthesis in plants is an example of what ? (a) excretion (b) irritability (c) nutrition (d) reproduction
Lee Reply
If a Hox 13 gene in a mouse was replaced with a Hox 1 gene, how might this alter animal development?
Israel Reply
Which of the following organisms is most likely to be a diploblast?
what are reactions of photosynthesis?
Maria Reply
what are the probabilities of blood genotypes for the offspring from a cross between a mother lAlA blood and a father with lBi blood?
dayana Reply
what is matter
Emmanuel Reply
matter is anything that has mass and can occupied space
example of matter
You serves as an example of matter Because matter is anything that has mass and occupy space e.g man and every other things that exist on earth.. So think of every other things around you ...
and you too
We generally
What is ecological management
how the kidney functions as osmoregulatory organ
Sam Reply
That true
what is the major connection for sugars in glycolysis?
Ibrahim Reply
Simple term of science
Palesa Reply
what does it mean
it's means what do u know about biology?
what is immunisation
the action of making a person immune to infections ,for immunisation
what is the biology? what do you know about biology
Phathu Reply
biology is the study of living organisms, divided into many specialized fields that cover their morphology, physiology, anatomy, behavior, origin, and distribution.
The study of all aspects of life. The study of all living organisms (such as animal cells and plant cells) in greater detail (their structure and how they function). It's a very broad science.
what is prokaryotic
Bhaskar Reply
what is pathogens
pathogens are a bacterium, virus, or other microorganism that can cause disease.
transistion metals....
Wasik Reply

Get the best Biology course in your pocket!

Source:  OpenStax, Biology. OpenStax CNX. Feb 29, 2016 Download for free at http://cnx.org/content/col11448/1.10
Google Play and the Google Play logo are trademarks of Google Inc.

Notification Switch

Would you like to follow the 'Biology' conversation and receive update notifications?