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Image acquisition

The optical-fiber system

We have designed an optical system to test the imaging capabilities of a one millimeter diameter optical fiber probe. We used a 455 nm Luxeon LED (Philips, San Jose, CA) as the illumination source in accordance with our design goal of a device capable of both reflectance and fluorescence imaging while remaining as simple as possible. A broadband light source would be more ideal for reflectance imaging. However, a broadband light source could perform fluorescence imaging whereas our source would be suitable for both fluorescence and reflectance imaging. The LED was cooled by a heat sink and fan assembly mounted on its back and powered by a 12V AC/DC adaptor. To mimic the fatty environment of the human breast, test images were taken from samples of chicken adipose tissue.

The 1-mm diameter fiber (Sumitomo Electric USA, Los Angeles, CA) was positioned next to the LED and over a vertically adjustable sample stage. (Figure 2) The fiber tip was adjusted to receive maximum illumination from the LED when it was in contact with the sample. The fiber collected the light reflected by the sample at its distal end and conveyed it to its proximal end. The proximal end of the fiber was focused at the focal plane of an infinity corrected microscope objective (Newport, Irvine, CA). The position of the fiber was adjusted by a three-axis micro translation stage (Newport). The infinity corrected object could project an image from its rear that is in focus regardless of the distance between the object and the detector.

The detector was a Zeiss MRc5 5.0 megapixels color CCD camera (Carl Zeiss, Thornwood, NY). The magnification of the image on the detector relative to the image on the proximal fiber tip was the ratio between the distance from the detector to the back of the objective and the distance from the front of the objective to the fiber proximal tip. The infinity corrected objective permitted a wide range of magnifications (a while range of allowable detector-objective separation) while maintaining the focus of the image. (Figure 3) This feature could potentially allow the incorporation of a movable detector that can change the magnification of the image on command.

The image from the detector was captured onto a laptop computer (Lenovo, China) with the Zeiss AxioVision camera software. (Figure 4) The image was exported as a bitmap file. The bitmap file was subsequently processed in Matlab (Mathworks, Natick, MA).

Questions & Answers

do you think it's worthwhile in the long term to study the effects and possibilities of nanotechnology on viral treatment?
Damian Reply
absolutely yes
how to know photocatalytic properties of tio2 nanoparticles...what to do now
Akash Reply
it is a goid question and i want to know the answer as well
characteristics of micro business
Do somebody tell me a best nano engineering book for beginners?
s. Reply
what is fullerene does it is used to make bukky balls
Devang Reply
are you nano engineer ?
fullerene is a bucky ball aka Carbon 60 molecule. It was name by the architect Fuller. He design the geodesic dome. it resembles a soccer ball.
what is the actual application of fullerenes nowadays?
That is a great question Damian. best way to answer that question is to Google it. there are hundreds of applications for buck minister fullerenes, from medical to aerospace. you can also find plenty of research papers that will give you great detail on the potential applications of fullerenes.
what is the Synthesis, properties,and applications of carbon nano chemistry
Abhijith Reply
Mostly, they use nano carbon for electronics and for materials to be strengthened.
is Bucky paper clear?
so some one know about replacing silicon atom with phosphorous in semiconductors device?
s. Reply
Yeah, it is a pain to say the least. You basically have to heat the substarte up to around 1000 degrees celcius then pass phosphene gas over top of it, which is explosive and toxic by the way, under very low pressure.
Do you know which machine is used to that process?
how to fabricate graphene ink ?
for screen printed electrodes ?
What is lattice structure?
s. Reply
of graphene you mean?
or in general
in general
Graphene has a hexagonal structure
On having this app for quite a bit time, Haven't realised there's a chat room in it.
what is biological synthesis of nanoparticles
Sanket Reply
what's the easiest and fastest way to the synthesize AgNP?
Damian Reply
types of nano material
abeetha Reply
I start with an easy one. carbon nanotubes woven into a long filament like a string
many many of nanotubes
what is the k.e before it land
what is the function of carbon nanotubes?
I'm interested in nanotube
what is nanomaterials​ and their applications of sensors.
Ramkumar Reply
what is nano technology
Sravani Reply
what is system testing?
preparation of nanomaterial
Victor Reply
Yes, Nanotechnology has a very fast field of applications and their is always something new to do with it...
Himanshu Reply
good afternoon madam
what is system testing
what is the application of nanotechnology?
In this morden time nanotechnology used in many field . 1-Electronics-manufacturad IC ,RAM,MRAM,solar panel etc 2-Helth and Medical-Nanomedicine,Drug Dilivery for cancer treatment etc 3- Atomobile -MEMS, Coating on car etc. and may other field for details you can check at Google
anybody can imagine what will be happen after 100 years from now in nano tech world
after 100 year this will be not nanotechnology maybe this technology name will be change . maybe aftet 100 year . we work on electron lable practically about its properties and behaviour by the different instruments
name doesn't matter , whatever it will be change... I'm taking about effect on circumstances of the microscopic world
how hard could it be to apply nanotechnology against viral infections such HIV or Ebola?
silver nanoparticles could handle the job?
not now but maybe in future only AgNP maybe any other nanomaterials
I'm interested in Nanotube
this technology will not going on for the long time , so I'm thinking about femtotechnology 10^-15
how did you get the value of 2000N.What calculations are needed to arrive at it
Smarajit Reply
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Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
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