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Introduction

Abstract

The nuclear-cytoplasmic ratio (NCR) is a widely used clinical indicator for cancer. The automated extraction of cell size and nuclear size from tissue images is the only practical method to assess quantitatively the NCR in a real-time clinical setting. In this study we demonstrate the feasibility of automatically detecting the cell boundaries and estimating the cell sizes from images of chicken adipose tissue using several image processing techniques and an implementation of the Hough Transform.

Goals

Using a completely non-invasive image capturing technique, we want to be able to automate a process to detect the cells in the image, as well as calculate the area of the cells. By doing this, when imaging techniques become more advanced, the process can be used to determine the NCR and thus, become an automated, non-invasive indicator of the presence of cancer.

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In biology, a pathogen (Greek: πάθος pathos "suffering", "passion" and -γενής -genēs "producer of") in the oldest and broadest sense, is anything that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a germ. The term pathogen came into use in the 1880s.[1][2
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Source:  OpenStax, Detection of cell boundaries in optical fiber probe images. OpenStax CNX. Jan 20, 2008 Download for free at http://cnx.org/content/col10501/1.2
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